Journal:

Article Title: Expression of E-Selectin, P-Selectin, and Intercellular Adhesion Molecule-1 during Experimental Murine Listeriosis

doi:

Figure Lengend Snippet: Immunohistochemical detection of ICAM-1 (A, B, D, F, I, J), P-selectin (C, E, G), and neutrophils (H) in animals infected with L. monocytogenes. A: ICAM-1 expression in the hepatic sinusoids, with marked intensity around a pyogranulomatous lesion. B: ICAM-1 de novo expression on biliary epithelial cells (asterisk) within an inflamed portal area. C: P-selectin expression on endothelial cells (arrows) and circulating platelets (asterisk) in a hepatic central vein adjacent to an inflammatory infiltrate. Note subendothelial cellular extravasation (arrowheads). Expression of ICAM-1 (D, F) and P-selectin (E, G) on meningeal vessels before (D, E) and after (F, G) the arrival of inflammatory cells. Note in D the expression of ICAM-1 in the meningeal connective tissue (arrowheads). H:Immunohistochemistry to detect neutrophils showing the inflammatory pattern in the CNS affecting the subarachnoid space, especially in the hippocampal sulcus (arrowheads), internal meninges (arrows), and ventricular compartments (star). I: Purulent choroiditis in a lateral ventricle showing an intense expression of ICAM-1 on choroid epithelial cells. J: de novo ICAM-1 expression on ependymal cells of an animal with ventriculitis. Inflammatory cells are seen in the ventricular lumen. Original magnification, ×214 (A, D, E, I); ×428 (B, C, F, G, J); ×128 (H). Hematoxylin counterstain.

Article Snippet: Primary rat monoclonal antibodies against mouse ICAM-1 (clone KAT-1) (R&D Systems, Abingdon, UK), E-selectin (clone 10E9C) (Pharmingen, San Diego, CA), neutrophils (clone 7/9) (Immunokontact, Bioggio, Switzerland) and polyclonal rabbit against mouse P-selectin (Pharmingen) were used.

Techniques: Immunohistochemical staining, Infection, Expressing, Immunohistochemistry